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Effect of Heteropterys aphrodisiaca on Anxiety and Male Exposure of Female Mice with Advanced Age

[ Vol. 12 , Issue. 2 ]

Author(s):

Daniela M. Farias*, Maria S. Ostetto, Rozimeri Colleti, Thais Barbosa, Jorge A. Barros, Sergio Machado, Eric Murillo-Rodríguez, Susana E. Moreno and Andre B. Veras Pages 106-112 (7)

Abstract:


Background: Effect of Heteropterys aphrodisiaca (dog-node) on anxiety and function of adult female wistar mice. The project is an experiment with the use of H. aphrodisiac root extract, in order to observe the frequency of sexual exposure of females exposed to the extract, quantify the effect of the extract on the concentration of total testosterone and observe the anxiety levels of the animals exposed. Results will be measured with the laboratory testosterone test and LCE and CA tests.

Methods: In preparation of the extract, the root was oven dried at 40°C and diluted in alcohol extract (100g of root for 1 liter of alcohol) and lyophilized. 40 adult female mice were enrolled, separated in control group (placebo) and treatment group (50 mg/kg/day) for 15, 30, 45 and 60 days. At each period, hormonal testosterone and anxiety levels by the Elevated-Cross Labyrinth (ECL) tests and Open Camp (CA) were measured in 10 animals that were later euthanized (SBNeC).

Results: The results showed an improvement in the decrease of anxiety, as shown in the variables of number of open arm entries, time on the same side of the field, less avoidance and leakage. However, it appears that the time of exposure to the extract does not result in increased benefit, with possible decline of effect after 45 days of use.

Conclusion: With this performed experiment with the “no-de-cachorro” extract, it was possible to understand a little more how this root can act in relation to anxiety, as predicted by the pharmacology that validates the animal models; anxiolytic components decrease anxiety-related behaviors, as shown in the variables of entry numbers in the open arm, time on the same side of the field, less avoidance and escape. However, it seems that the time of exposure to the extract does not modify the performance in the tests, observing until an apparent exhaustion of the anxiolytic action, which evidences the need for more specific studies on the possible effects of the extract.

Keywords:

H. aphrodisiaca, root, extract, anxiety, open field, elevated cross labyrinth.

Affiliation:

Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS, Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS, Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS, Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS, Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS, Laboratory of Panic and Respiration, Institute of Psychiatry, Federal University of Rio de Janeiro (IPUB/UFRJ), Rio de Janeiro, Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina Division Ciencias de la Salud, Universidad Anahuac Mayab, Merida, Yucatan, Post-Graduation Program in Biotechnology, Dom Bosco Catholic University, Campo Grande-MS, Post-Graduation Program in Health Psychology, Dom Bosco Catholic University, Campo Grande-MS

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